I. DESCRIPTION: The U.S. Environmental Protection Agency, Research Triangle Park (RTP) Animal Resources Program Office (ARPO) has a requirement for a replacement sterilizer in the RTP vivarium A580 decontamination room. The A580 sterilizer supports the on-going research performed in the Building A animal laboratories. The current sterilizer is a Steris Amsco Eagle 3000SL, double door, through-wall Steam Sterilizer (A580/A580A). The replacement sterilizer shall fit into the existing footprint, wall opening and bioseal space with no additional construction required for tear out or installation. The replacement sterilizer shall have approximately the same throughput capability as the existing sterilizer. The unit will be used to sterilize dirty cages, dirty bedding, and associated waste from studies using primarily BSL2 pathogens. II. Salient Characteristics: The contractor shall provide one (1) sterilizer/autoclave with the following characteristics: • Shall be able to fit into existing wall opening and bioseal space without any new construction. Current bioseal measures 42X76 inches, the current bioseal frame measures 44X77 inches. • Shall be currently available in the marketplace for the government to buy. • Shall fit in existing sterilizer footprint and still be able to receive preventative maintenance and servicing. • Double door, through wall construction with either vertical (preferred) or hinged left hand opening. Shall have capability to sterilize both solids and liquids. • Capable of operation utilizing building supplied steam. Building pressure set point is 70 psi, but pressure at unit is decreased due to distance from source. • Capable of operation utilizing building supplied potable water. • Autoclave should include a backflow prevention device. • Sterilizer shall use electric power • Have an insulated sterilization chamber of sufficient size to sterilize mouse caging (19″L x 10-1/2″W x 6″D) and rat caging (19″L x 10″W x 8 1/2″D). • Certified and stamped as meeting Underwriters Laboratories Standard 61010-1, Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use – Part 1: General Requirements • Certified and stamped as meeting ASME Boiler and Pressure Code Section VIII Division 1. • Shall be provided with preprogrammed cycles meeting sterilization requirements for infectious waste as required State of North Carolina regulations including a gravimetric, prevacuum, and liquid sterilization cycle. • Control system permits storage of at least 5 preprogrammed cycles. • Controls provide the option of requiring an access code or other identification method to operate the sterilizer or change cycle parameters. • Controls shall be located so as not to expose the sensitive components to steam and heat. • Controls shall be capable of providing a printed record of all pertinent cycle data including key transition points in the cycle and alarms and deviations that may jeopardize the sterilization process. • Pressure gauges displaying the chamber and jacket pressure accessible to the operator. • An operators manual. • The following safety features and alarms, if possible: o Chamber Emergency Manual Exhaust Valve – allows the operator to vent the chamber and save the product in case of power outage. o Chamber door seal interlock and alarm – prevent cycle starting until the door is sealed and aborts the cycle and safely exhausts the chamber if door seal is lost during the cycle. Interlock should also prevent the door from being opened until chamber is vented to a safe pressure. o Excessive condensate/vessel flooding – alarm and interlock to abort the cycle and vent the chamber safely if excessive condensate is sensed within the chamber. o Pressure relief valve – prevent chamber pressure greater than rated pressure. o Under Temperature Alarm – informs the operator that the temperature has dropped the sterilization temperature. o Over Temperature Alarm – informs the operator that the chamber temperature has gone above a set point. o Waste line alarm – informs the operator that drain line temperature is outside the normal range. o Pressure/temperature alarm – informs the operator that chamber pressure or temperature readings are outside the normal steam range during sterilize phase. |